Journal: Heliyon

Article Title: Dihydroartemisinin modulated arachidonic acid metabolism and mitigated liver inflammation by inhibiting the activation of 5-LOX and COX-2

doi: 10.1016/j.heliyon.2024.e33370

Figure Lengend Snippet: DHA decreased the expression of PLIN2, COX-2 and 5-LOX in liver. IHC analysis of PLIN2, COX-2, and 5-LOX in the liver after DHA treatment in Yap1 LKO mice. Sections were microscopically observed at 20 × and 40 × magnification. n ≥ 4, * P < 0.05.

Article Snippet: Primary antibodies included YAP (D8H1X) XP rabbit monoclonal antibody (14074S, CST, 1:200), ADRP/Perilipin 2 (PLIN2) rabbit polyclonal antibody (15,294-1-AP, Proteintech, 1:250), Cyclooxygenase 2 (COX-2) rabbit monoclonal antibody (CY8852, Abways, 1:100), and 5 Lipoxygenase (5-LOX) rabbit monoclonal antibody (CY8019, Abways, 1:100).

Techniques: Expressing

Journal: Obesity (Silver Spring, Md.)

Article Title: Intramyocellular Lipid Droplet Size Rather Than Total Lipid Content Is Related to Insulin Sensitivity After 8-Weeks of Overfeeding

doi: 10.1002/oby.21980

Figure Lengend Snippet: A) Perilipin 3 (PLIN3) protein content decreased with overfeeding (p=0.04, N=27). B) Baseline human primary myotubes treated with 200μM of palmitate showed that PLIN2 is expressed later over a time course of 2 hours. C) Changes in PLIN2 protein content correlated inversely with glucose disposal rate (GDR, r=-0.43, p=0.03, N=25). Graphs represent mean±S.E.M, *p<0.05.

Article Snippet: Antibodies against Total Akt (Cat no. 9272), p-S473 Akt (Cat no. 9271), Total IRS1 (Cat no. 2382S), p-S1101 IRS1 (Cat no. 2385), and mTOR (Cat no. 4517) were obtained from Cell Signaling Technology (Danvers, MA) The antibodies for PLIN2 (Cat no. NB110-40877) and PLIN3 (Cat no. NB110-40764) were obtained from Novus Biologicals (Littleton, CO).

Techniques:

Journal: Cell reports

Article Title: RalA and PLD1 promote lipid droplet growth in response to nutrient withdrawal

doi: 10.1016/j.celrep.2021.109451

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Mouse monoclonal anti-ADRP (B-6) (Perilipin2) , Santa Cruz Biotechnology , Cat# sc-377429.

Techniques: Recombinant, Software, Microscopy